Coding
Part:BBa_K5042231:Design
Designed by: Tzu-Yu Huang Group: iGEM24_Taipei-KCISLK-V2 (2024-09-28)
Anabaena Sensory Rhodopsin (ASR) is a photoreceptor that regulates expression through the sensation
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 30
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Throughout the light induction experiment, the ASR is inserted in the pET-17b-ASR, and the Flag-tag is fused with ASR protein as the ASR inhibits the expression of genes in the dark. In specific, when the C-terminus of ASR binds to the DNA, in this case, is the BBa_K2596001 above, ASR will inhibit gene expression.
Source
The Anabaena Sensory Rhodopsin(ASR) is tested and applied throughout the light induction experiment by team Taipei_KCISLK_V2 in the lab.
Presenters & Abstracts | Cal Poly Humboldt. (2024). Cal Poly Humboldt. https://pse.is/6gvk4j